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Encompassing Amino Acids: 35-320
Application: Useful for binding studies.
Assay Conditions: The protein was validated by ELISA for binding to CEACAM1. Different amounts of CD66b, Avi-His-Tag Recombinant (#102028) were used to coat a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with PBST and blocked with 100 µl/well of Blocking Buffer 7 for 90 min at Room Temperature (RT). After removing the blocking buffer, 50 ng of diluted CEACAM, Avi-His-Tag, Biotin Labeled Recombinant (#70201) was added for 60 minutes at RT. The plate was washed 3 times, blocked, and incubated with Streptavidin-HRP, followed by washing, and incubation with HRP luminescence substrate. Chemiluminescence is proportional to CEACAM1 binding to CEACAM8.
Background: CD66b (cluster of differentiation 66b), also known as CEACAM8 (Carcinoembryonic antigen-related cell adhesion molecule 8), belongs to the CEA family of proteins and the immunoglobulin superfamily, and is expressed in neutrophils, eosinophils, granulocytes, and monocytes. It was initially described as a granulocyte marker, but recent studies have shown that it is involved in cell adhesion and is a pro-inflammatory mediator. It is highly glycosylated and binds to GPI (glycosylphosphatidylinositol). CD66b+ monocytes can be found in several cancer types and represent a population of cells that do not seem to be involved in immunosuppression but display high phagocytic activity and co-stimulate T cell proliferation and IFN-γ secretion. CD66b+ neutrophils are also present in the tumor microenvironment (TME) and are believed to link to a poor prognosis. Anti-CD66b antibodies have been used in the case of ADCs (antibody drug conjugate).
Description: Recombinant human CD66b, also known as CEACAM8 (Carcinoembryonic antigen-related cell adhesion molecule 8), encompassing amino acids 35-320. This construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_001816.4
Purity: ≥90%
Supplied As: At least 6 months at -80°C.
Tags: CD67 antigen, Carcinoembryonic antigen CGM6, Non-specific cross-reacting antigen NCA-95, CEACAM8
Target: C-terminal Avi-Tag and His-Tag
Uniprot: P31997
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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