CD73, His-Tag (Human) Recombinant

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Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.

Assay Conditions: Assay was done in 50 µL assay buffer containing 40 mM Tris, pH 7.4, 5 mM MgCl2, 0.1 mg/ml BSA and 40 µM AMP for 20 min at 37°C. Inorganic phosphate produced was measured using Biomol Green (Enzo Life Science) reagent.

Description: Human CD73, also known as 5'- nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_002526, a.a. 27-547 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 58.6 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

Genbank: NM_002526

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: P21589

Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.
2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.

• Artikelnummer: BPS71184-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 71184-1
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK