CDK1/CyclinB1, GST-Tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for studying enzyme kinetics and screening inhibitors.

Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 µM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Human CDK1/CyclinB1complex, also known as CDC and CCNB1,CCNB respectively. GenBank AccessionNos. NM_001786 and NM_031966respectively, full-length with N-terminal GST-tagson both proteins, expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 60 kDa (CDK1) MW = 75 kDa(CyclinB1).

Format: Aqueous buffer solution

Formulation: 40 mM Tris pH 8.0, 110 mMNaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mMDTT and 20% glycerol.

Genbank: CDK1: NM_001786; CyclinB1: NM_031966

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: CDK1: P06493; CyclinB1: P14635

Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Vantieghem, A. et al., J. Biol. Chem. 2002; 277(40): 37718-37731.
2. Rigas, A.C. et al., Oncogene. 2007; 26(55): 7611-7619.

• Artikelnummer: BPS40454
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40454
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect