CDK12/Cyclin K Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Background: The CDK12/Cyclin K complex comprises human CDK12 (Cyclin Dependent Kinase 12) and human Cyclin K. Cyclin-dependent kinase (CDK) complexes have been implicated in the regulation of transcription. The CDK12/ Cyclin K complex regulates phosphorylation of Serine 2 in the C-terminal domain of RNA polymerase II, which is responsible for productive transcriptional elongation and synthesis of full-length mature mRNAs. This complex contributes to the maintenance of genome stability and plays a role in embryonic development as well as cancer.

Description: The CDK12/ Cyclin K Kinase Assay Kit is designed to measure the activity of complex CDK12/ Cyclin K for screening and profiling applications using luminescent Kinase-Glo® MAX as a detection reagent.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Zhang, T., Kwiatkowski, N., Olson, C. et al.Covalent targeting of remote cysteine residues to develop CDK12 and CDK13 inhibitors. Nat Chem Biol 12, 876-884 (2016). https://doi.org/10.1038/nchembio.2166
2. Bösken CA, Farnung L, Hintermair C, Merzel Schachter M, Vogel-Bachmayr K, Blazek D, Anand K, Fisher RP, Eick D, Geyer M. The structure and substrate specificity of human Cdk12/Cyclin K. Nat Commun. 2014 Mar 24;5:3505. doi: 10.1038/ncomms4505. PMID: 24662513; PMCID: PMC3973122.

• Artikelnummer: BPS78298
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78298
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK