CDK17/Cyclin Y, GST-tags Recombinant

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Encompassing Amino Acids: CDK17: 1-523(end)
Cyclin Y: 2-341(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 200 µg of MBP (or Histone H1) as substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Description: CDK17/Cyclin Y complex of human CDK17, also known as PCTK2, CDK17, PCTAIRE2, GenBank Accession No. NM_002595, a.a. 1-523(end) with N-terminal GST-tag, MW=88 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.

Formulation: 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: CDK17: NM_002595; Cyclin Y: NM_145012

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: CDK17: N-terminal GST-tag
Cyclin Y: N-terminal GST-tag

Uniprot: CDK17: Q00537
Cyclin Y: Q8ND76

Warnings: Avoid freeze/thaw cycles. 

Biosafety Level: Not applicable (BSL-1)

References: 1. Meyerson, M. et.al, EMBO J. 11: 2909-2917, 1992.
2. Hirose T. et.al, Eur J Biochem. 1997 Oct 15;249(2):481-8.

• Artikelnummer: BPS100606
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100606
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK