CDK2/CyclinA2, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 µM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Recombinant full-length human CDK2 (GenBank Accession No. NM_001798), a.a. 2-298 (end) with N-terminal GST-tag, MW = 61 kDa, and CyclinA2 (GenBank Accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 75 kDa. Both proteins were coexpressed in a baculovirus Sf9 insect cell expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_001798 (CDK2), NM_001237 (CyclinA2)

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P24941 (CDK2), Q13825 (CyclinA2)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9.
2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

• Artikelnummer: BPS40101
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40101
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect