CDK2 (no tag)/CyclinA2, His-GST-tags Recombinant

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Encompassing Amino Acids: CDK2 a.a. 1-298 (end), CyclinA2 a.a. 174-432 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 µM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).

Description: Complex of recombinant full-length human CDK2 (GenBank accession No. NM_001798), a.a. 1-298 (end), MW = 34 kDa with no tag, and CyclinA2 (GenBank accession No. NM_001237), a.a. 174-432 (end) with N-terminal His-GST-tags, MW = 57 kDa. Both were co-expressed in a baculovirus Sf9 insect cell expression system.

Format: Aqueous buffer solution

Formulation: 20 mM NaH₂PO₄/Na₂HPO₄, pH 8.0, 400 mM NaCl, 20 mM glutathione, 10% glycerol, 2 mM DTT, and 1 mM EDTA

Genbank: NM_001798 (CDK2), NM_001237 (CyclinA2)

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: CDK2 no tag, CyclinA2 N-terminal His-GST-tags

Uniprot: P24941 (CDK2), P20248 (CyclinA2)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9.
2. Hara KT., et al., Dev Biol. 2005 Oct 1,286(1):102-13.

• Artikelnummer: BPS41101
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 41101
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect