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CDK5/p35, GST-tag Recombinant
CDK5/p35, GST-tag Recombinant
Artikelnummer
BPS40095
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in Kinase buffer containing 0.2 mM DTT using Histone 1 substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
Background: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
Description: Recombinant full-length human CDK5 (59 kDa) and human p35 (60 kDa), both with N-terminal GST-tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.
Genbank: NM_004935 (CDK5), NM_003885 (p35)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q00535 (CDK5), Q15078 (p35)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tang, D. et al. Prog Cell Cycle Res. 1996,2:205-16.
2. Dhavan, R. et al. Nat Rev Mol Cell Biol. 2001 Oct,2(10):749-59.
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in Kinase buffer containing 0.2 mM DTT using Histone 1 substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
Background: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
Description: Recombinant full-length human CDK5 (59 kDa) and human p35 (60 kDa), both with N-terminal GST-tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.
Genbank: NM_004935 (CDK5), NM_003885 (p35)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q00535 (CDK5), Q15078 (p35)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tang, D. et al. Prog Cell Cycle Res. 1996,2:205-16.
2. Dhavan, R. et al. Nat Rev Mol Cell Biol. 2001 Oct,2(10):749-59.
| Artikelnummer | BPS40095 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40095 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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