CDK6/CyclinD3, His-tags Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 M ATP at 30°C for 60 min. The amount of ATP transferred was calculated using Kinase- Glo reagent (Promega).

Description: Complex of N-terminal Histaggedrecombinant full-length humanCDK6 (GenBank Accession No.X66365), and N-terminal His tagged,recombinant full length, Cyclin D3.(GenBank Accession No. M90814).Coexpressed in a Baculovirus infectedSf9 cell expression system. CDK6MW=38 kDa, Cyclin D3 MW=33 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, 110 mMNaCl, 2.2 mM KCl, 300 mM Imidazole, 3mM DTT and 20% glycerol

Genbank: NM_001259/NM_001760

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tags

Uniprot: Q00534 (CDK6); P30281 (Cyclin D3)

Warnings: Avoid freeze/thaw cycles. Storing diluted enzymeis not recommended.

Biosafety Level: Not applicable (BSL-1)

References: 1. Gauduchon J. et al., Int J Cancer. 2008 May 1,122(9):2130-41.
2. Faast R. et al., Oncogene. 2004 Jan 15,23(2):491-502.

• Artikelnummer: BPS40206
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40206
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect