Ceramide Kinase, FLAG-tag Recombinant

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Encompassing Amino Acids: 1-537(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl₂, 10 mM MgCl₂, 2 mM DTT, 5 µM ATP) using a beta-Octylglucoside mixed micellar assay with 100 µM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI).

Description: Human ceramide kinase (CERK), GenBank Accession No. NM_022766, a.a. 1-537(end) with C-terminal FLAG-tag, MW= 60 kDa, expressed in a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 ug/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Genbank: NM_022766

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal FLAG-tag

Uniprot: Q8TCT0

Unit Definition: A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Mitra,P. et al., FEBS Lett. 581 (4), 735-740 (2007)
2. Kim,T.J. et al., Biochem. Biophys. Res. Commun. 342 (2), 611-617 (2006)

• Artikelnummer: BPS40617
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40617
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect