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Chemi-Verse™ CDK8/Cyclin C Kinase Assay Kit
Chemi-Verse™ CDK8/Cyclin C Kinase Assay Kit
Artikelnummer
BPS78886
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: CDK8 (cyclin dependent kinase 8) is a member of the cyclin-dependent protein kinase family involved in cell cycle regulation, transcription and splicing. CDK associates with cyclins, which induce a conformational change that results in a dramatic increase of the kinase activity. Cyclin levels vary during the cell cycle, which allows cyclins to regulate CDK activity in the cell. Dissociation of the complex returns CDK to its basal activity, and CDK is degraded by ubiquitin mediated proteolysis. In addition to cyclin, CDK8 is part of a Mediator complex that includes CDK19, MED12 and MED13 and acts as a negative regulator of RNA Polymerase II. CDK8 regulates several pathways, such as Notch, Wnt/β-catenin and Sonic Hedgehog (Shh) and has been described as an oncogenic driver and implicated in several cancer types (breast, ovarian, gastric cancer and leukemia). The understanding of the mechanisms involved in cell cycle regulation, and its control via the use of small molecule inhibitors will open new therapeutic avenues for the treatment of cancer.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ CDK8/Cyclin C Kinase Assay is a luminescent assay designed to measure CDK8 (cyclin-dependent kinase 8)/Cyclin C kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK8/Cyclin C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: CDK8: P49336: CyclinC: P24863
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: CDK8 (cyclin dependent kinase 8) is a member of the cyclin-dependent protein kinase family involved in cell cycle regulation, transcription and splicing. CDK associates with cyclins, which induce a conformational change that results in a dramatic increase of the kinase activity. Cyclin levels vary during the cell cycle, which allows cyclins to regulate CDK activity in the cell. Dissociation of the complex returns CDK to its basal activity, and CDK is degraded by ubiquitin mediated proteolysis. In addition to cyclin, CDK8 is part of a Mediator complex that includes CDK19, MED12 and MED13 and acts as a negative regulator of RNA Polymerase II. CDK8 regulates several pathways, such as Notch, Wnt/β-catenin and Sonic Hedgehog (Shh) and has been described as an oncogenic driver and implicated in several cancer types (breast, ovarian, gastric cancer and leukemia). The understanding of the mechanisms involved in cell cycle regulation, and its control via the use of small molecule inhibitors will open new therapeutic avenues for the treatment of cancer.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ CDK8/Cyclin C Kinase Assay is a luminescent assay designed to measure CDK8 (cyclin-dependent kinase 8)/Cyclin C kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK8/Cyclin C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: CDK8: P49336: CyclinC: P24863
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS78886 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 78886 |
| Green Labware | Nein |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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