Chemi-Verse™ PARP7 Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Study enzyme kinetics and screening small molecule inhibitors for drug discovery and HTS applications.

Contraindications: The Chemi-Verse™ PARP7 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.

Description: The Chemi-Verse™ PARP7 Assay Kit is an ELISA-type chemiluminescent assay designed to measure PARP7 enzymatic activity for screening and profiling applications. PARP7 catalyzes the NAD-dependent ADP-ribosylation of histones. This 96-well or 384-well format assay kit contains sufficient amounts of purified PARP7 enzyme (amino-acids 400- 657), histone mixture, and PARP assay buffer for 100 or 400 enzyme reactions. The Chemi-Verse™ PARP7 Assay Kit takes advantage of a highly specific ADP-ribose binding reagent. First, histone proteins are coated on a 384-well plate. Next, an NAD⁺ substrate is incubated with the PARP7 enzyme in an optimized assay buffer. Finally, the plate is treated with the ADP-ribose specific binding reagent and secondary HRP-conjugated antibody, followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.Figure 1. Chemi-Verse™ PARP7 chemiluminescent assay schematic.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: This 96-well format assay kit contains sufficient amounts of purified PARP7 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

Uniprot: Q7Z3E1

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78814-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78814-1
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK