Chemi-Verse™ RSK4 Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Background: RSK4 (ribosomal S6 kinase 4) is a member of the serine/threonine RSK family of proteins. In vertebrates this family has four isoforms, RSK1-4, involved in cell growth, proliferation, motility, and apoptosis, acting as downstream effectors of the Ras/ERK (extracellular signal regulated kinase) pathway. RSK4 is found at high levels in the kidney, the brain, and the thyroid gland. It is linked to ESCC (esophageal squamous cell carcinoma) where it is found at high levels and phosphorylates GSK-3β to activate the β-catenin signaling pathway and leads to resistance to radiotherapy. Inhibition of this protein with the inhibitor BI-D1870 was able to increase the sensitivity of the ESCC cells to radiotherapy in animal models. In glioblastoma RSK4 phosphorylates EZH2 (enhancer of zeste homolog 2) and contributes to resistance to treatment with EZH2 inhibitors. RSK4 contributes to treatment resistance and cancer progression in kidney carcinoma, melanoma and RCC (renal cell carcinoma) cell lines, but the role of RSK4 as a tumor suppressor or oncogene may be cancer dependent and remains controversial. A deeper understanding of the role of this protein in cancer combined with the use of inhibitors alone or in combinatory therapy may prove a new therapeutic avenue in cancer therapy.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Chemi-Verse™ RSK4 Kinase Assay Kit is designed to measure RSK4 (ribosomal S6 kinase 4) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified RSK4, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: Q9UK32

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Li M.-Y., et al., 2020 J Clin Invest 130 (8) : 4301-4319.
Pang F., et al., 2023 Cancer Gene Ther. 30(12) :1636-1648.

• Artikelnummer: BPS82523
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82523
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK