Purification Method: Egg yolk-derived purified IgY (non affinity purified).
Source: Chickens were immunized with 2 peptides (CGKKTRGGNKASDSGT, CKRPPPKKDREPTTKRPKL), each conjugated to KLH. After multiple immunizations, eggs were collected and IgY was purified from the egg yolk. The antibody was NOT affinity purified.
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide.
References: 1. Upton, JW, van Dyck, LF and SH Speck. 2005. Characterization of murine gammaherpesvirus 68 v-cyclin interactions with cellular cdks. Virology 341:271-283.
2. Herskowitz, JH, Siegal, AM, Jacoby, MA and SH Speck. 2008. Systematic Mutagenesis of the Murine Gammaherpesvirus 68 M2 Protein Identifies Domains Important for Chronic Infection. J. Virol. 82: 3295-3310.
3. Aligo J., Brosnan K, Walker M, Mikkelson ER, Burleson GR, Burleson FG, Volk A, Weinstock D, 2014. Murine gammaherpesvirus-68 (MHV-68) is not horizontally transmitted amongst laboratory mice by cage contact. J. Immunotoxicol. Nov. 21:1-12
4. Santana AL Oldenburg DG, Kirillov V, Malik L, Dong Q, Sinayev R, Marcu KB, White DW, Krug LT. RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency. Pathogens. 2017 Feb 16;6(1). pii: E9. doi: 10.3390/pathogens6010009RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency. Pathogens. 2017 Feb 16;6(1). pii: E9. doi: 10.3390/pathogens6010009
2. Qiwen Dong, Kyle R. Smith, Darby G. Oldenburg, Maxwell Shapiro, William R. Schutt, Laraib Malik, Joshua B. Plummer, Yunxiang Mu, Thomas MacCarthy, Douglas W. White, Kevin M. McBride, Laurie T. Krug
Combinatorial Loss of the Enzymatic Activities of Viral Uracil-DNA Glycosylase and Viral dUTPase Impairs Murine Gammaherpesvirus Pathogenesis and Leads to Increased Recombination-Based Deletion in the Viral Genome.
American Society for Microbiology, 2018, DOI: 10.1128/mBio.01831-18.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.