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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: CHEK2 was incubated with a substrate for 1h at RT in kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Description: Human Checkpoint kinase 2 (GenBank Accession No. NM_007194), full length, with N-terminal His-tag. MW=91 kDa. Expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 40% glycerol and 3 mM DTT.
Genbank: NM_007194
Host Cell Line: Sf9 cells
Storage Stability: >12 months at -80°C
Tags: N-terminal His-tag
Uniprot: O96017
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Reinhardt HC and Yaffe MB. Curr Opin Cell Biol. 2009 Apr;21(2):245-55.
2. Stracker TH and Petrini JH. Cell Cycle. 2008 Dec,7(23):3618-21.