Application Note: Anti-CIITA antibody has been tested in western blot. For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications.
Concentration Value: 90 mg/mL
ELISA Dilution: 1:5,000 - 1:25,000
Western Blot Dilution: 1:500 - 1:3,000
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Immunogen: The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-CIITA antibody was prepared from monospecific antiserum by delipidation and defibrination.
Background: Anti-CIITA antibody detects CIITA. The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.
Low Endotoxin: No
Other: User Optimized