cKit (D816V), His-tag Recombinant

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Encompassing Amino Acids: 544-976(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Tyr Peptide 6, Invitrogen) at 30°C for 1 hour.

Description: HumancKit (D816V) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of aspartic acid 816 to valine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Genbank: NM_000222

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P10721

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.

• Artikelnummer: BPS40252
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40252
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect