cKit (V654A), His-tag Recombinant

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Encompassing Amino Acids: 544-976(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 4 µg /ml recombinant cKit. Assay was done with 0.2 mg/ml Poly-(Glu-Tyr) peptide using ADPGlo® (Promega) conditions at 30°C for 35 minutes.

Description: Human Kit (V654A) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of valine654 to alanine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Genbank: NM_000222

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P10721

Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.

• Artikelnummer: BPS40251
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40251
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect