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Claudin-18 Isoform 2, FLAG-Tag Recombinant
Claudin-18 Isoform 2, FLAG-Tag Recombinant
Artikelnummer
BPS101570-1
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The protein was validated by measuring its binding to Anti-Claudin-18 Isoform 2 IgG Antibody (BPS Bioscience #101564) in biochemical binding (ELISA) assay. The Claudin-18 Isoform 2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified Anti-Claudin-18 Isoform 2 IgG Antibody (BPS Bioscience #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with HRP Colorimetric Substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Description: Recombinant human Claudin-18, Isoform 2 (CLDN 18), full length, encompassing amino acids 1-261(end). This construct contains a C-terminal Flag-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 50 mM HEPES, pH 8, 500 mM NaCl, 10% glycerol, 0.1% DDM, 0.01% CHS, and 100 µg/ml FLAG peptide
Genbank: NM_001002026
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: P56856
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The protein was validated by measuring its binding to Anti-Claudin-18 Isoform 2 IgG Antibody (BPS Bioscience #101564) in biochemical binding (ELISA) assay. The Claudin-18 Isoform 2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified Anti-Claudin-18 Isoform 2 IgG Antibody (BPS Bioscience #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with HRP Colorimetric Substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Description: Recombinant human Claudin-18, Isoform 2 (CLDN 18), full length, encompassing amino acids 1-261(end). This construct contains a C-terminal Flag-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 50 mM HEPES, pH 8, 500 mM NaCl, 10% glycerol, 0.1% DDM, 0.01% CHS, and 100 µg/ml FLAG peptide
Genbank: NM_001002026
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: P56856
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101570-1 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101570-1 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Human |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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