Claudin-4 Lentivirus

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Applications: Generate stable cell line expressing human Claudin-4 with puromycin selection

Background: Claudins are integral membrane proteins and major components of tight junction strands which serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Claudin-4 is one of the 27 members of the Claudin family.Claudin-4 mediates paracellular chloride transport and plays an important renal function through the reabsorption of filtered chloride. The CLDN4 gene is deleted in Williams-Beuren syndrome, leading to multi-systemic neurodevelopmental disorders. The protein acts as a high-affinity receptor for Clostridium perfringens enterotoxin (CPE) during infection by gram-positive Clostridial bacteria, which are responsible for food poisoning and gastrointestinal illnesses. Recent studies indicate that the protein may be overexpressed in ovarian cancer.

Description: The Claudin-4 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human Claudin-4 (NM_001305.5) driven by an EF1A promoter and a puromycin selection marker (Figure 1).Figure 1: Schematic of the lenti-vector used to generate the Claudin-4 Lentivirus

Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Uniprot: O14493

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• Artikelnummer: BPS78723
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78723
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK