Claudin-9 Lentivirus

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Applications: Generate stable cell line expressing human Claudin-9 with puromycin selection

Background: Claudins are integral membrane proteins and major components of tight junction strands which serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Claudin-9 is one of the 27 members of the Claudin family.Claudin-9 is overexpressed in hepatocellular carcinoma, where it enhances the metastatic potential of hepatocytes via Tyk2/Stat3 signaling. Claudin-9 is also a marker for endometrial cancer, and acts as a receptor for Clostridium perfringens enterotoxin (CPE) during infection by gram-positive Clostridial bacteria. C. perfringens infection causes for food poisoning and gastrointestinal illnesses, via Claudin-9 to toxin-induced gut barrier breakdown.

Description: The Claudin-9 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human Claudin-9 (NM_020982) driven by an EF1A promoter and a puromycin selection marker (Figure 1).Figure 1: Schematic of the lenti-vector used to generate the Claudin-9 Lentivirus.

Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Uniprot: O95484

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• Artikelnummer: BPS78721
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78721
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: STK