CSK, GST-His-Th-Tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30µg/ml Poly (Glu:Tyr)4:1 substrate, and 1 µg/ml recombinant CSK.

Description: Human CSK (GenBank Accession No. NM_004383), full length with N-terminal GST-tag, MW= 80 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, 10 mM reduced glutathione, and 3 mM DTT.

Genbank: NM_004383

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P41240

Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Manjarrez-Orduño N, et al. Nat Genet. 2012 Nov,44(11):1227-30.
2. de la Puerta ML, et al. PLoS One. 2013,8(1):e54569.

• Artikelnummer: BPS40410
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40410
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect