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CTLA4:B7-1(Biotinylated) Inhibitor Screening Assay Kit
CTLA4:B7-1(Biotinylated) Inhibitor Screening Assay Kit
Artikelnummer
BPS72009
Verpackungseinheit
96 rxns.
Hersteller
BPS Bioscience
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Applications: This kit is useful for screening for inhibitors of B7-1 binding to CTLA4.
Background: The activation of naÍve T cells requires two signals, the specific T cellreceptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC),and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cellsurface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in aninhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an importantdrug target for the regulation of the host's response to cancer.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt.
Description: The CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CTLA4 is coated on a 96-well plate. Next, B7-1 is incubated with CTLA4 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CTLA4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Uniprot: P33681
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Linsley, P.S., et al. J. Exp. Med. 1991, 174(3): 561-569.
2. Hurwitz, A.A., et al. Canc. Res. 2000, 60: 2444-2448.
Applications: This kit is useful for screening for inhibitors of B7-1 binding to CTLA4.
Background: The activation of naÍve T cells requires two signals, the specific T cellreceptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC),and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cellsurface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in aninhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an importantdrug target for the regulation of the host's response to cancer.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt.
Description: The CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CTLA4 is coated on a 96-well plate. Next, B7-1 is incubated with CTLA4 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CTLA4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Uniprot: P33681
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Linsley, P.S., et al. J. Exp. Med. 1991, 174(3): 561-569.
2. Hurwitz, A.A., et al. Canc. Res. 2000, 60: 2444-2448.
| Artikelnummer | BPS72009 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 72009 |
| Green Labware | Nein |
| Verpackungseinheit | 96 rxns. |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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