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Encompassing Amino Acids: 36-162
Application: Useful for studying protein binding and screening small molecular inhibitors
Description: Human secreted CTLA4, Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36- 162, fused at the C-terminus to the Fc portion of human IgG1 and with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein was biotinylated using AviTag™ technology. MW = 42 kDa (monomer). Note: This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_005214
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
Uniprot: P16410
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dilek, N., et al., PLOS ONE. 2013 Dec. 23; 8(12):e83139.
2. Leach, D.R., et al., Science. 1996; 271(5256):1734-1736.
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