Cul5 Antibody

Application Note: Anti-Cul5 has been tested by immunohistochemistry. This antibody reacts with human Cul5 by western blot and immunoprecipitation.  The antibody immunoprecipitates protein from cell lysates (using 293T, U2OS and others).  This antibody also co-immunoprecipitates associated proteins.  A 90.9 kDa band corresponding to human Cul5 is detected.  Most cell lines expressing Cul4A can be used as a positive control.  Researchers should determine optimal titers for other applications.

Concentration Value: 85 mg/mL

ELISA Dilution: 1:2,000 - 1:10,000

Immunohistochemistry Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:1,000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 1-25 of Human Cul5 (N-terminus) coupled to KLH.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 5.  Cross reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor.  Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC100-401-A05
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-A05
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Immunoprecipitation, Western Blotting, Immunohistochemistry
• Isotyp: Antiserum
• Human Gene ID: 8065
• Wirt: Rabbit
• Konjugat: Unconjugated