Cyclin B1 Antibody

Application Note: Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.

Concentration Value: 80 mg/mL

ELISA Dilution: 1:2,000 - 1:10,000

Immunohistochemistry Dilution: 1:500

Immunoprecipitation Dilution: 1:100

Western Blot Dilution: 1:500 - 1:1,000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination.  Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.  No reaction was observed against other related cyclins.  Cross reactivity with Cyclin B1 from other species may also occur.

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC100-401-152
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-152
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: Western Blotting, Immunohistochemistry
• Isotyp: Antiserum
• Human Gene ID: 891
• Wirt: Rabbit
• Konjugat: Unconjugated