Cyclin B1(CCNB1/1098), 1mg/mL

Description: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Product Origin: Animal - Mus musculus (mouse)

Clone: CCNB1/1098

Entrez Gene ID: 891

Immunogen: Recombinant human full-length CCNB1 protein

Verified AB Applications: Flow (verified)/IF (verified)/IHC (FFPE) (verified)

Z-Antibody Applications: Flow (verified)/IF (verified)/IHC, FFPE (verified)

• Artikelnummer: BTMBNUM1098-50
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUM1098-50
• Green Labware: Nein
• Verpackungseinheit: 50 uL
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Flow Cytometry, Immunohistochemistry
• Isotyp: IgG1 kappa
• Wirt: Mouse