Application Note: Anti-Cyclin-E antibody is suitable for the detection by immunoblot, ELISA and IP. Researchers should determine optimal titers for applications that are not stated below. Antiserum will specifically react with a 45 kDa Cyclin E protein from human tissue.
Concentration Value: 70 mg/mL
ELISA Dilution: 1:100,000
Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:2,000 - 1:10,000
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Immunogen: Anti-Cyclin-E Antibody was produced by repeated immunizations with a KLH conjugated peptide corresponding to a region near the carboxy terminus of human Cyclin E.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Cyclin-E was prepared from monospecific antiserum by delipidation and defibrination. No reaction was observed against other related cyclins. Cross reactivity with Cyclin E from other species may also occur.
Background: Anti-Cyclin-E antibody detects cyclin-E. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-cyclin-e Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Low Endotoxin: No
Other: User Optimized