Cyno CD8a/CD8b Lentivirus

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Application: Study CD8-MHC class I interaction.Generate Cyno CD8a and CD8b-expressing cell pools or stable cell lines following puromycin selection.

Background: CD8 (cluster of differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that functions within the immune system to mediate cell-cell interactions. The functional forms of CD8 consist of either a heterodimer of two isoforms, CD8α and CD8β, or a homodimer of two CD8α molecules. CD8 acts as a coreceptor to facilitate binding between the T cell Receptor (TCR) and the class I Major Histocompatibility Complex (MHC).  Studying CD8 and its importance in isoimmunity can further our understanding of post-transplant recognition.

Description: Cyno CD8a/CD8b Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a P2A-linked cynomolgus monkey (Macaca fascicularis) CD8a (XP_065382910.1) and CD8b (XP_005575417.1) driven by an EF1A promoter, and a puromycin selection marker.

Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Uniprot: CD8a: P01732; CD8b: P10966

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• Artikelnummer: BPS82373
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82373
• Verpackungseinheit: 500 µl x 2
• Mengeneinheit: PAK