Cytochrome C (Mitochondrial Marker)(7H8.2C12), 1mg/mL

Description: Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Product Origin: Animal - Mus musculus (mouse)

Clone: 7H8.2C12

Entrez Gene ID: 54205

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Verified AB Applications: IHC (FFPE) (verified)/WB (verified)

Z-Antibody Applications: IHC, FFPE (verified)/WB (verified)

• Artikelnummer: BTMBNUM0183-50
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUM0183-50
• Green Labware: Nein
• Verpackungseinheit: 50 uL
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Various species, Frog (Xenopus Laevis), Horse (Equine), Fruit Fly (Drosophila Melanogaster)
• Klonalität: Monoclonal
• Methode: Western Blotting, Immunohistochemistry
• Isotyp: IgG2b kappa
• Wirt: Mouse