Cytokeratin, HMW(AE-3), 1mg/mL

Description: This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, BSA-free

Concentration: 1 mg/mL

Storage buffer: PBS, no BSA, no azide

Product Origin: Animal - Mus musculus (mouse)

Clone: AE-3

Entrez Gene ID: 51350

Immunogen: Human epidermal keratin

Expected AB Applications: Flow (intracellular)/IHC (frozen) (published for clone)/IF (published for clone)/WB (published for clone)

Verified AB Applications: IHC (FFPE) (verified)

Z-Antibody Applications: Flow, intracellular/IF (published)/IHC, frozen (published)/IHC, FFPE (verified)/WB (published)

• Artikelnummer: BTMBNUM0257-50
• Hersteller: Biotium
• Hersteller Artikelnummer: BNUM0257-50
• Green Labware: Nein
• Verpackungseinheit: 50 uL
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Monkey (Primate), Rabbit, Dog (Canine), Cow (Bovine), Chicken
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Immunohistochemistry (frozen), Western Blotting, Flow Cytometry, Immunohistochemistry
• Isotyp: IgG1 kappa
• Wirt: Mouse