DCAMKL2, GST-tag Recombinant

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Encompassing Amino Acids: 1-690

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: DCAMKL2 (also known as doublecortin-like kinase 2 or DCLK2) is a close homologue of doublecortin or DCX which is associated with intractable epilepsy in humans, due to a severe disorganization of the neocortex and hippocampus known as classical lissencephaly. DCX; DCLK2-null mice display frequent spontaneous seizures that originate in the hippocampus, with most animals dying in the first few months of life. DCX and DCLK2 are co-expressed in developing hippocampus, and, in their absence, there is dosage-dependent disrupted hippocampal lamination associated with a cell-autonomous simplification of pyramidal dendritic arborizations leading to reduced inhibitory synaptic tone (1).

Description: Recombinant human DCAMKL2 (a.a. 1-690), with N-terminal GST-tag, expressed by baculovirus in Sf9 cells

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_001040261

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q8N568

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Kerjan G. Et al: Mice lacking doublecortin and doublecortin-like kinase 2 display altered hippocampal neuronal maturation and spontaneous seizures. Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6766-71.

• Artikelnummer: BPS40048
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40048
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect