iLite® ADCC Target CD20 (-) Assay Ready Cells are depleted of CD20 expression, to be used as a control for unspecific activation of ADCC that can be a confounding factor when performing ADCC assays. iLite ADCC Target CD20 (-) Assay Ready Cells are based on a human B lymphocyte cell line, Raji (ATCC# CCL-86), and have been genetically engineered and optimized to be depleted of CD20 expression. The cells are to be use as negative controls together with iLite® ADCC Effector (V) Assay Ready Cells and iLite® ADCC Target CD20 (+) Assay Ready Cells for measuring the ADCC activity of anti-CD20 antibodies.