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Application: Expression of human DLL4 in cells of interest.Generation of cell pools or stable cell lines expressing human DLL4 following puromycin selection.
Background: Notch signaling is an evolutionarily conserved pathway that plays a critical role in cell fate decisions and differentiation. A member of the Delta family of Notch ligands, DLL4 (delta-like 4) is a vascular transmembrane protein which signals through its corresponding receptors Notch1 and Notch4 and plays a critical role in embryonic development, where it is essential for both vascular development and T-cell specification. DLL4 expression is induced by both VEGF (vascular endothelial growth factor) and hypoxia. Once bound to its receptor, DLL4 induces the cleavage and translocation of NICD (notch intracellular domain) to the nucleus, where it activates the expression of target genes. It plays an important role in blood vessel growth by regulating the differentiation of tip and stalk cells directing vascular sprouting. Early studies of the role of DLL4-Notch signaling in tumor angiogenesis demonstrated that inhibition of DLL4 decreased tumor growth. DLL4 has been implicated in atherosclerosis, cancer, and inflammatory disorders. More recently, a role in M1 macrophage polarization and inflammatory cytokine expression has been identified. DLL4 is an attractive therapeutic target with several clinical trials of DLL4 inhibitors ongoing, including trials of bispecific antibodies targeting both DLL4 and VEGF.
Description: DLL4 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human DLL4 (delta like canonical notch ligand 4) (NM_019074.4), driven by a CMV promoter, and a puromycin selection marker.
Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: Q9NR61
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Yuwen Y., et al., 2023 Heliyon 9(10): e20777.