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DNA Damage (8-OHdG) ELISA Kit
Colorimetric detection of 8-hydroxy-2-deoxy Guanosine
Artikelnummer
STRSKT-120-96S
Verpackungseinheit
96 well
Hersteller
Stressmarq Biosciences
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Target: DNA Damage (8-OHdG)
Product Type: ELISA Kits
Trademark: MOLECULAR SIGNATURE®
Detection Method: Colorimetric Assay
Assay Type: Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Utility: ELISA Kit for 8-OHdG detection in samples.
Precision: Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.
Sample Type(s): Urine,Cell Lysates,Plasma,Sample matrices
Number of Samples: 39 samples in duplicate
Incubation Time: 1 hour
Sensitivity: 0.59 ng/mL
Assay Range: 0.94 - 60 ng/mL
Specificity: Cross-reactivity: 8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.
Scientific Background: 8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
References: 1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120.2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34.3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids.4. Lin H., et al. (2004) Biochem J. 380: 541-548.5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666.6. Lee J., et al. (2005) Hypertension 45: 986-990.7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152.8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75.9. Kuo H., et al. (2007) Mutat Res. 631:62-68.10. Shen J., et al. (2007) Cancer 109: 574-580.11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636.12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110.13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236.14. Floyd R.A. (1990) FASEB J 4: 2587-2597.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Product Type: ELISA Kits
Trademark: MOLECULAR SIGNATURE®
Detection Method: Colorimetric Assay
Assay Type: Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Utility: ELISA Kit for 8-OHdG detection in samples.
Precision: Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.
Sample Type(s): Urine,Cell Lysates,Plasma,Sample matrices
Number of Samples: 39 samples in duplicate
Incubation Time: 1 hour
Sensitivity: 0.59 ng/mL
Assay Range: 0.94 - 60 ng/mL
Specificity: Cross-reactivity: 8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.
Scientific Background: 8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
References: 1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120.2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34.3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids.4. Lin H., et al. (2004) Biochem J. 380: 541-548.5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666.6. Lee J., et al. (2005) Hypertension 45: 986-990.7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152.8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75.9. Kuo H., et al. (2007) Mutat Res. 631:62-68.10. Shen J., et al. (2007) Cancer 109: 574-580.11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636.12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110.13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236.14. Floyd R.A. (1990) FASEB J 4: 2587-2597.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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