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DNMT3A/DNMT3L Complex, His-tag, GST-tag Recombinant
DNMT3A/DNMT3L Complex, His-tag, GST-tag Recombinant
Artikelnummer
BPS51106
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-1632
Applications: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS Bioscience DNMT3A Assay Kit, Cat. #52033. Add 50 µl reaction mixcontaining DNMT3A/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection.
Description: Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
Genbank: NM_175629(DNMT3A), NM_013369 (DNMT3L)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His, N-terminal GST-tag
Uniprot: Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Encompassing Amino Acids: 2-1632
Applications: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS Bioscience DNMT3A Assay Kit, Cat. #52033. Add 50 µl reaction mixcontaining DNMT3A/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection.
Description: Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
Genbank: NM_175629(DNMT3A), NM_013369 (DNMT3L)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His, N-terminal GST-tag
Uniprot: Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS51106 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 51106 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
