DNMT3B/DNMT3L Complex, GST-tag, His-tag Recombinant

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Encompassing Amino Acids: 564-853(end), DNMT3b; 160-387(end), DNMT3L

Applications: Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

Assay Conditions: Assayed using BPS Bioscience DNMT3B Assay Kit, Cat. #52034. Add 50 µl reaction mixcontaining DNMT3B/DNMT3L to wells coatedwith DNMT substrate. Incubate 2 hr at 37°C.Add antibody against 5-methylcytosine, thenHRP-labeled secondary antibody followed bychemiluminescent detection.

Description: Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal GST-tag, MW=59 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal His-tag, MW = 27 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerol

Genbank: NM_006892(DNMT3B), NM_013369 (DNMT3L)

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST (DNMT3B) N-terminal His-tag (DNMT3L)

Uniprot: Q9UBC3 (DNMT3B), Q9UJW3 (DNMT3L)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS51109
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 51109
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect