DOT1L (KMT4) Chemiluminescent Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Description: The DOT1L Chemiluminescent Assay Kit is designed to measure DOT1Lactivity for screening and profiling applications. The key to the DOT1L Chemiluminescent Assay Kit is a highly specific antibody thatrecognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiterplate are required for methyltransferase detection. First, S-adenosylmethionine is incubated witha sample containing assay buffer and methyltransferase enzyme for two hours. Next, primaryantibody is added. Finally, the strips are treated with an HRP-labeled secondary antibodyfollowed by addition of HRP substrate to produce chemiluminescence that can then bemeasured using a chemiluminescence reader.

Storage Stability: One year from date of receipt when stored as directed.

Supplied As: The DOT1L Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate, DOT1L substrate, primary antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Min, J., et al. Cell 2003; 112:711-723.
2. Daigle, S.R., et al. Cancer Cell 2011, 20:53-65.

• Artikelnummer: BPS52202
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 52202
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK