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Encompassing Amino Acids: 2-416
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human DOT1L (GenBank Accession No. NM_032482), a.a. 2-416 with N-terminal GST-tag, MW = 73 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol.
Genbank: NM_032482
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q15002
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. McGinty, R.K. et al., Nature 453 (7196), 812-816 (2008).
2. Steger, D.J. et al., Mol. Cell. Biol. 28 (8), 2825-2839 (2008).