DYRK1B, GST-tag Recombinant

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Encompassing Amino Acids: 2-629(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was done in Kinase buffer containing DYRKtide substrate peptide 1 (0.2 mg/ml) and
20 µM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B (DYRK1B), also known as Minibrain-Related Kinase (MIRK), GenBank Accession No. NM_004714, a.a. 2-629(end), with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 95 kDa.

Formulation: 40 mM Tris-HCl, pH 8.0. 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% Glycerol and 3 mM DTT.


Genbank: NM_004714

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal GST-tag

Uniprot: Q9Y463

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Mao, J. et al., JBC. 277(38): 35156-35161, 2002.
2. Guimera, J. W. et al., Genomics. 57(3): 407-418, 1999.

• Artikelnummer: BPS100646
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100646
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK