eGFP/Firefly Luciferase K562 Cell Line

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Applications: In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging.Use as negative control in CAR-T cell co-culture experiments.Use as target cells in NK co-culture killing assays

Background: The K562 cell line was established from a patient with chronic myelogenous leukemia (CML) in terminal blast crisis. The K562 Cell Line does not express antigens CD19, CD20 or CD22, therefore it makes an excellent negative control in assays that measure specific killing by CAR-T cells targeting B cell antigens CD19, CD20, and CD22. It can also be used as a highly sensitive in vitro target in natural killer assays. The presence of eGFP and luciferase allows for easy assay readouts, making this cell line a convenient choice.

Description: The eGFP/Firefly Luciferase K562 Cell Line are engineered human lymphoblast K562 cells expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. The cells were generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741), which is a SIN (self-inactivating) lentivirus.

Host Cell Line: K562, human lymphoblast cell line, suspension.

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ~1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

• Artikelnummer: BPS78911
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78911
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human