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EGFR, GST-tag, His-tag, Recombinant
EGFR, GST-tag, His-tag, Recombinant
Artikelnummer
BPS40187
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 668 -1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in a kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 30 min. ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human EGFR (GenBank Accession No. NM_005228), a.a. 668-1210 with N-terminal GST-tag, MW=87.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol and 1 mM DTT.
Genbank: NM_005228
Host Cell Line: Sf9 cells
Purity: ≥70%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-Tag
Uniprot: P00533
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nakamura, J.L. Expert Opin. Ther. Targets 11 (4), 463-472 (2007).
2. Kim, H.J., et al. Curr. Biol. 17 (5), 455-461 (2007).
Application Reference: Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).Product Specific Reference(s):
1. Mowafy, S., et al., Eur J Med Chem. 61, 132-45 (2013).
Encompassing Amino Acids: 668 -1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in a kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 30 min. ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human EGFR (GenBank Accession No. NM_005228), a.a. 668-1210 with N-terminal GST-tag, MW=87.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol and 1 mM DTT.
Genbank: NM_005228
Host Cell Line: Sf9 cells
Purity: ≥70%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-Tag
Uniprot: P00533
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nakamura, J.L. Expert Opin. Ther. Targets 11 (4), 463-472 (2007).
2. Kim, H.J., et al. Curr. Biol. 17 (5), 455-461 (2007).
Application Reference: Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).Product Specific Reference(s):
1. Mowafy, S., et al., Eur J Med Chem. 61, 132-45 (2013).
| Artikelnummer | BPS40187 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40187 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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