EGFR (T790M, L858R), GST-tag, His-tag Recombinant

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Encompassing Amino Acids: 668-1210

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Human EGFR T790M L858R (GenBank Accession No. NM_005228), a.a. 668-1210 with substitutions of Threonine790 to Methionine and Leucine858 to Arginine and an N-terminal GST-tag, MW= 88 kDa, expressed in a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 8.5, 250 mM NaCl, 10% glycerol and 2 mM DTT.

Genbank: NM_005228

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag, C-terminal His-Tag

Uniprot: P00533

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007)
2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007).
Application Reference:
Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).

• Artikelnummer: BPS40350
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40350
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect