EHMT1 (GLP), GST-tag Recombinant

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Encompassing Amino Acids: 894-1298 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and EHMT1 (0.05 - 0.5 ng)) is added to substrate-coated wells on a Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Description: Human euchromatic histone-lysine N-methyltransferase (EHMT1), also known as G9a-like protein, GLP or KMT1D, GenBank Accession No. NM_024757, a.a. 894-1298 (end) with N-terminal GST-tag, MW= 72.4 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT

Genbank: NM_024757

Host Cell Line: E. coli

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q9H9B1

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.
2. Ueda, J., et al., J Biol. Chem. 2006 Jul 21; 281(29): 20120-8.

• Artikelnummer: BPS51020
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 51020
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK