EIF2AK2, GST-tag Recombinant

EIF2AK2, GST-tag Recombinant
Artikelnummer
BPS40058
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience

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Encompassing Amino Acids: 252-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: EIF2AK2 (also known as double-stranded RNA-activated protein kinase) is a protein kinase that has been shown to be involved in HIV/gp120-associated neurodegeneration (1). EIF2AK2 acts as a critical mediator of gp120 neurotoxicity and is a substrate for a family of protein kinases that respond to various forms of environmental stress. Activation of EIF2AK2 leads to its autophosphorylation and then phosphorylation of its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2. EIF2AK2 plays a critical role in mRNA translation, cell proliferation and apoptosis. A novel cross-talk between the EIF2AKs and p53 has been shown that has implications in cell proliferation and tumorigenesis (2).

Description: Recombinant human EIF2AK2 (a.a. 252-end), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_002759

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P19525

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Baltzis, D. et al: The eIF2alpha kinases PERK and PKR activate glycogen synthase kinase 3 to promote the proteasomal degradation of p53. J. Biol Chem. 2007; 282(43):31675-87.
2. Alirezaei, M. et al: Human immunodeficiency virus-1/surface glycoprotein 120 induces apoptosis through RNA-activated protein kinase signaling in neurons. J. Neurosci. 2007;27(41):11047-55.
Mehr Informationen
Artikelnummer BPS40058
Hersteller BPS Bioscience
Hersteller Artikelnummer 40058
Green Labware Nein
Verpackungseinheit 10 µg
Mengeneinheit STK
Wirt Insect
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