EPHA2, His-tag Recombinant

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Encompassing Amino Acids: 596-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 ?M Na orthovanadate, 0.1 mM ATP, 30 ?g/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 ?g /ml recombinant EPHA2.

Description: Human EPHA2 (GenBank Accession No. NM_004431), a.a. 596-end with N-terminal His-tag, MW=47 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.

Genbank: NM_004431

Host Cell Line: Sf9 cells

Storage Stability: >12 months at -80°C

Tags: N-terminal His-tag

Uniprot: P29317

Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Nakada,M., et al., Cancer Res. 64 (9), 3179-3185 (2004).
2. Kataoka,H.,et al., J. Cancer Res. Clin. Oncol. 128 (7), 343-348 (2002).

• Artikelnummer: BPS40190
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40190
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect