EPHB2, His-tag Recombinant

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Encompassing Amino Acids: 569-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Invitrogen Zlyte Peptide 1) at room temperature for 1 hour.

Description: Human EPHB2 (GenBank Accession No. NM_004442), a.a. 569-end with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.

Genbank: NM_004442

Host Cell Line: Sf9 cells

Storage Stability: >12 months at -80°C

Tags: N-terminal His-tag

Uniprot: P29323

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Macrae,M., et al., Cancer Cell 8 (2), 111-118 (2005).
2. Xu,H., et al., Am. J. Physiol. Renal Physiol. 288 (4), F855-F866 (2005).

• Artikelnummer: BPS40200
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40200
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect