Notes: EZ Cap™ Cas9 mRNA encodes for Cas9, a DNA endonuclease that induces breaks in DNA sequences complementary to single-guide RNA (sgRNA) strands and is commonly used for genome editing.{71100} It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap™ Cas9 mRNA is also polyadenylated and modified with 5-methyoxy-UTP (mo5U) to reduce the host cell immune response and enhance mRNA stability. Encapsulation of EZ Cap™ Cas9 mRNA (mo5U) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of Cas9 in vitro or in vivo.{68856}