Notes: EZ Cap™ Cre mRNA encodes for Cre, a bacteriophage tyrosine recombinase that catalyzes homologous recombination between two loxP sites in DNA.{65824} It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap™ Cre mRNA is also polyadenylated and modified with N1-methylpseudouridine (m1Ψ) to reduce the host cell immune response and enhance mRNA stability. Encapsulation of EZ Cap™ Cre mRNA (m1Ψ) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of Cre in vitro or in vivo.{66852}