Formulation: 1 mM Sodium citrate, pH 6.4
Shelf life (days): 180
Notes: EZ Cap™ mCherry mRNA encodes for mCherry, a red fluorescent protein and derivative of Discosoma red fluorescent protein (DsRed) that displays excitation and emission maxima of 587 and 610 nm, respectively.{49803} It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap™ mCherry mRNA is also polyadenylated and modified with N1-methylpseudouridine (m1Ψ) to reduce host cell immune response and enhance mRNA stability. Encapsulation of EZ Cap™ mCherry mRNA (m1Ψ) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of mCherry fluorescent protein in vitro or in vivo.{72450}